| Detection principle

The Two Main Routes of Multiple Nucleic Acid Testing

Route 1: Each tube amplifies 1 to 4 xN tubes and synchronously performs optical detection;

Route 2: Single tube amplification with a weight of 10 to 100+amplification followed by hybridization, electrophoresis, and first or second generation sequencing detection.

| Core patents

| How do we achieve multiplicity?

26 reaction chambers combined with 2-color fluorescence channels to achieve 52 fold detection

| How do we achieve rapid amplification？

●Ultra-thin wall thickness: The wall thickness of our chip is only 40um, which is 1/5 of the conventional PCR tube, so we can improve the thermal conductivity fivefold.

●Large surface area: The chip thickness is only 1.2mm, the chip has a large surface area.

●Heating and cooling on both sides: Both sides of the chip are heated, detects from the side, so the liquid in the chip can get faster heating and cooling rate and better temperature evenness.

●Super detection speed：Combined with fast reaction enzyme, DNA amplification can be completed in 18 minutes and RNA amplification can be completed   in 23 minutes.

| How do we achieve storage and transportation with no cold chain?

Conventional molecular diagnostic reagents are liquid, which must be transported and stored in the cold chain (-20 ° C), and the storage and transportation cost is high. Conventional molecular diagnostic systems usually add a variety of PCR enhancers and cryoprotectants, to improve the sensitivity, specificity and fidelity of PCR amplification, such as betaine, dimethyl sulfoxide, formamide, glycerol, PEG, semen glue and single strand DNA binding protein, but many components will affect the drying performance of the amplification system. We remove ingredients that affect drying, such as glycerin, from the system, and select enzymes and other reaction raw materials, and strictly control the drying process to achieve long-term storage of premixed nucleic acid system (except all components of nucleic acid template) at room temperature.

| Why are our products easy to use？

1.The reaction system is premixed and dried on the chip, and there is no need to configure the reaction system separately

2.Sample input port is connected to the reaction chamber through the flow channel. When the user injects the nucleic acid template, the reagent is automatically distributed to each chamber to complete the construction of the reaction system. Less on-site liquid dispensing operation, the possibility of aerosol pollution is greatly reduced.

| Comparison